Application

Western Blotting Analysis: 10 µg/mL from a representative lot detected BAP1 in whole cell extracts of HEK293T cells over-expressing BAP1 protein. (Courtesy of Hongzhuang Peng, Ph.D. and Frank J. Rauscher III, Ph.D., The Wistar Institute, Philadelphia PA USA).

Western Blotting Analysis: A representative lot detected BAP1 in Western Blotting applications (Abdel-Wahab, O., et. al. (2012). Cancer Cell. 22(2):180-93).

Immunoprecipitation Analysis: A representative lot detected BAP1 in Immunoprecipitation applications (Abdel-Wahab, O., et. al. (2012). Cancer Cell. 22(2):180-93).

Anti-BAP1 Antibody, clone 3C11, Cat. No. MABE1069, is a highly specific mouse monoclonal antibody that targets BAP1 and has been tested in and Immunoprecipitation and Western blotting.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ubiquitin carboxyl-terminal hydrolase BAP1 (EC 3.4.19.12; UniProt Q92560; also known as BRCA1-associated protein 1, Cerebral protein 6) is encoded by the BAP1 (also known as KIAA0272, hucep-6) gene (Gene ID 8314) in human. Originally identified and named as a BRCA1-interacting protein, BAP1 is a tumor suppressor that participates in DNA-damage response, DNA synthesis and cell cycle progression. BAP1 belongs to the ubiquitin C-terminal hydrolase (UCH) family of deubiquitin enzymes (DUBs), all members of which, including UCH-L1, UCH-L3 and UCH-L5, are characterized by a conserved UCH catalytic domain (CD; BAP1 a.a. 1-235). BAP1 and UCH-L5 also share a conserved C-terminal ULD domain (a.a. 640-710 of BAP1). The region between BAP1 CD and ULD is important for binding to the HCF-1 protein. In addition, BAP1 contains a C-terminal extension (CTE; a.a. 711-729) following its ULD. Study shows that while CTE tethers BAP1 to the nucleosome core, additional interaction with ASXL1 DEUBiquitinase ADaptor domain (DEUBAD) is needed to allow BAP1 substrate processing by increasing it′s affinity towards ubiquitin. In a similar fashion, UCH-L5 activity is reugulated via interaction with DEUBAD of RPN13 and INO80G. BAP1 is implicated in transcription regulation due to its interaction with transcription-related proteins, such as FOXK2, HCF-1, OGT, LSD2, MBD-6, MBD-5, ASXL1 and ASXL2. BAP1 loss leads to a variety of cancers, including metastatic cutaneous and uveal melanoma, pleural mesothelioma, renal cell carcinoma and bladder tumours.

Immunogen

GST-tagged full length recombinant human ubiquitin carboxyl-terminal hydrolase BAP1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HEK293T cells overexpressing BAP1.

Western Blotting Analysis: A 1:125 dilution of this antibody detected BAP1 in 10 µg of lysate from HEK293T cells overexpressing BAP1.

Specificity

Clone 3C11 specifically detects BAP1 in human. It targets an epitope within amino acids 337-440 in human BAP1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 80 kDa observed; 80.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).